CYBERMED LIFE - ORGANIC  & NATURAL LIVING

Cybermedlife - Therapeutic Actions Light-Emitting Diodes (LEDs) Therapy

LED enhances anti-inflammatory effect of. 📎

Abstract Title: LED enhances anti-inflammatory effect of. Abstract Source: Am J Transl Res. 2018 ;10(1):283-291. Epub 2018 Jan 15. PMID: 29423013 Abstract Author(s): Shengnuo Fan, Ahsan Habib, Jun Liu, Jun Tan Article Affiliation: Shengnuo Fan Abstract: Neuroinflammation is a complex pathological process usually results from abnormal microglial activation, thus, intervention in a microglial stimulation pathway could be a promising approach for the treatment of neurodegenerative diseases. Luteolin is an important bioflavonoid possesses anti-inflammatory properties, which is widely studied over these years. Light emitting diode (LED) therapy is reported to be a potential therapeutic strategy for many diseases including neurodegenerative diseases. However, little is known about the anti-inflammatory effect of LED therapy on activated microglial cells, even less is known whether there is a synergistic anti-inflammatory effect exist in LED and luteolin therapy. In this study, we aimed to confirm the anti-inflammatory effect of luteolin and LED combination therapy in lipopolysaccharide (LPS)-stimulated BV2 microglial cells. We showed that luteolin inhibited LPS-induced cytotoxicity, tumor necrosis factor alpha (TNFα) and interleukin-6 (IL-6) production through modulation of p38 and extracellular signal-regulated kinase (ERK) signaling in BV2 cells. In addition, LED therapy enhanced the anti-inflammatory effect of luteolin. These results suggest that a synergistic effect between luteolin and LED could be a new effective therapy in relieving neuroinflammation. Article Published Date : Dec 31, 2017

Photodynamic therapy of human biliary cancer cell line using combination of phosphorus porphyrins and light emitting diode.

Abstract Title: Photodynamic therapy of human biliary cancer cell line using combination of phosphorus porphyrins and light emitting diode. Abstract Source: Bioorg Med Chem. 2017 Oct 21. Epub 2017 Oct 21. PMID: 29108834 Abstract Author(s): Jin Matsumoto, Kou Suzuki, Masahide Yasuda, Yuya Yamaguchi, Yoshitaka Hishikawa, Naoya Imamura, Atsushi Nanashima Article Affiliation: Jin Matsumoto Abstract: A series of phosphorus porphyrin complexes ([(RO)2P(tpp)]Cl, tpp = tetraphenylporphyrinato group, R = -(CH2CH2O)m(CH2)nH; 1a: m = 2, n = 2; 1b: m = 2, n = 4; 1c: m = 2, n = 6; 1d: m = 3, n = 6) were used for the photodynamic therapy (PDT) of human biliary cancer cell line (NOZ) when exposed to the irradiation of light emitting diodes (LEDs). A Dulbecco's modified Eagle's medium (DMEM) containing NOZ cells (2000 cell well(-1)) and 1 (0-100 nM) was introduced into a 96-well microplate and incubated for 24 h to accumulate 1 into the NOZ cells and to multiply the NOZ cells until the cell number reached 10(4) cells well(-1). After replacing the DMEM medium containing 1 with a fresh DMEM medium without 1, the plates were irradiated for 30 min at 610 nm. After incubation was performed for 24 h in dark conditions, the cell viability of the NOZ cells was determined using the MTT assay. The half maximuminhibitory concentrations 50 (IC50) of 1a-1d were found to be in the range of 33.7-58.7 nM for NOZ. These IC50 values for the NOZ were one hundredth the IC50 value (7.57 μM) for mono-l-aspartyl chlorin e6 (laserphyrin®). Thus, it was found that the PDT activity of 1a-1d was much higher than the mono-l-aspartyl chlorin e6. Similarly, IC50 vales of 1a-1d for HeLa cells were found to be 27.8-52.5 nM. This showed that 1a-1d had high photodynamic activity in cancer cells. At the same time, it was speculated that an LED is a useful light source for deactivating the cancer cells because it can excite the sensitizers with peak width in their absorption spectra using the light of the specified wave length with band width of 10-20 nm; LEDs provide a homogeneous light distribution for the target cells. Article Published Date : Oct 20, 2017

Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells.

Abstract Title: Photobiomodulation therapy promotes neurogenesis by improving post-stroke local microenvironment and stimulating neuroprogenitor cells. Abstract Source: Exp Neurol. 2017 Oct 19. Epub 2017 Oct 19. PMID: 29056360 Abstract Author(s): Luodan Yang, Donovan Tucker, Yan Dong, Chongyun Wu, Yujiao Lu, Yong Li, Juan Zhang, Timon Cheng-Yi Liu, Quanguang Zhang Article Affiliation: Luodan Yang Abstract: Recent work has indicated that photobiomodulation (PBM) may beneficially alter the pathological status of several neurological disorders, although the mechanism currently remains unclear. The current study was designed to investigate the beneficial effect of PBM on behavioral deficits and neurogenesis in a photothrombotic (PT) model of ischemic stroke in rats. From day 1 to day 7 after the establishment of PT model, 2-minute daily PBM (CW, 808nm, 350mW/cm(2), total 294J at scalp level) was applied on the infarct injury area (1.8mm anterior to the bregma and 2.5mm lateral from the midline). Rats received intraperitoneal injections of 5-bromodeoxyuridine (BrdU) twice daily (50mg/kg) from day 2 to 8 post-stoke, and samples were collected at day 14. We demonstrated that PBM significantly attenuated behavioral deficits and infarct volume induced by PT stroke. Further investigation displayed that PBM remarkably enhanced neurogenesis and synaptogenesis, as evidenced by immunostaining of BrdU, Ki67, DCX, MAP2, spinophilin, and synaptophysin. Mechanistic studies suggested beneficial effects of PBM were accompanied by robust suppression of reactive gliosis and the production of pro-inflammatory cytokines. On the contrary, the release of anti-inflammatory cytokines, cytochrome c oxidase activity and ATP production in peri-infarct regions were elevated following PBM treatment. Intriguingly, PBM could effectively switch an M1 microglial phenotype to an anti-inflammatory M2 phenotype. Our novel findings indicated that PBM is capable of promoting neurogenesis after ischemic stroke. The underlying mechanisms may rely on: 1) promotion of proliferation and differentiation of internal neuroprogenitor cells in the peri-infarct zone; 2) improvement of the neuronal microenvironment by altering inflammatory status and promoting mitochondrial function. These findings provide strong support for the promising therapeutic effect of PBM on neuronal repair following ischemic stroke. Article Published Date : Oct 18, 2017

Photobiomodulation Therapy Alleviates Tissue Fibroses Associated with Chronic Graft-Versus-Host Disease: Two Case Reports and Putative Anti-Fibrotic Roles of TGF-β.

Abstract Title: Photobiomodulation Therapy Alleviates Tissue Fibroses Associated with Chronic Graft-Versus-Host Disease: Two Case Reports and Putative Anti-Fibrotic Roles of TGF-β. Abstract Source: Photomed Laser Surg. 2017 Oct 19. Epub 2017 Oct 19. PMID: 29053051 Abstract Author(s): Joel B Epstein, Judith E Raber-Durlacher, Marie-Charlotte Huysmans, Maria C E Schoordijk, Jerry E Cheng, Rene-Jean Bensadoun, Praveen R Arany Article Affiliation: Joel B Epstein Abstract: OBJECTIVE: Patients who receive allogeneic hematopoietic stem cell transplantation may experience oral complications due to chronic graft-versus-host disease (cGVHD). The manifestations may include progressive sclerosis-like changes that may involve various body sites, including the oropharynx. METHODS AND RESULTS: We present two cGVHD cases of oropharyngeal fibrotic changes that affected functions that were treated with photobiomodulation (PBM) therapy. These case reports suggest that PBM therapy represents an additional, innovative approach affecting discrete phases in cGVHD-associated fibrotic changes. CONCLUSIONS: We discuss these observations in the context of currently understood molecular mechanisms, especially induction of transforming growth factor beta and NFκB that appear to be counter-intuitive to their known roles in matrix synthesis and inflammation that contribute to tissue fibroses. The clinical benefit noted in the two cases presented clearly indicates that there are distinct mechanistic and biological insights in the regulation of these molecular pathways in determining therapeutic efficacy with PBM therapy. Article Published Date : Oct 18, 2017

Combinational light emitting diode-high frequency focused ultrasound treatment for HeLa cell.

Abstract Title: Combinational light emitting diode-high frequency focused ultrasound treatment for HeLa cell. Abstract Source: Comput Assist Surg (Abingdon). 2017 Sep 28:1-7. Epub 2017 Sep 28. PMID: 28956464 Abstract Author(s): Se-Woon Choe, Kitae Park, Chulwoo Park, Jaemyung Ryu, Hojong Choi Article Affiliation: Se-Woon Choe Abstract: PURPOSE: Light sources such as laser and light emitting diode or ultrasound devices have been widely used for cancer therapy and regenerative medicines, since they are more cost-effective and less harmful than radiation therapy, chemotherapy or magnetic treatment. Compared to laser and low intensity ultrasound techniques, light emitting diode and high frequency focused ultrasound shows enhanced therapeutic effects, especially for small tumors. MATERIALS AND METHODS: We propose combinational light emitting diode-high frequency focused ultrasound treatment for human cervical cancer HeLa cells. Individual red, green, and blue light emitting diode light only, high frequency focused ultrasound only, or light emitting diode light combined with high frequency focused ultrasound treatments were applied in order to characterize the responses of HeLa cells. RESULTS: Cell density exposed by blue light emitting diode light combined with high frequency focused ultrasound (2.19 ± 0.58%) was much lower than that of cells exposed by red and green light emitting diode lights (81.71 ± 9.92% and 61.81 ± 4.09%), blue light emitting diode light (11.19 ± 2.51%) or high frequency focused ultrasound only (9.72 ± 1.04%). CONCLUSIONS: We believe that the proposed combinational blue light emitting diode-high frequency focused ultrasound treatment could have therapeutic benefits to alleviate cancer cell proliferation. Article Published Date : Sep 27, 2017

Chemoresistance to 5-FU inhibited by 635 nm LED irradiation in CD133+ KB cell line.

Abstract Title: Chemoresistance to 5-FU inhibited by 635 nm LED irradiation in CD133+ KB cell line. Abstract Source: Lasers Med Sci. 2017 Sep 27. Epub 2017 Sep 27. PMID: 28956217 Abstract Author(s): Donghwi Kim, Mineon Park, Hyunwoong Jang, Hoon Hyun, Wonbong Lim Article Affiliation: Donghwi Kim Abstract: Consistent with cancer stem cell theory, a small fraction of cancer cells, described as cancer stem cells (CSCs), may promote tumor recurrence and anti-cancer drug resistance. Therefore, much effort has been devoted to the development of CSC targeted therapy to vanquish drug resistance. In this study, we have investigated the effect of multiple light-emitting diode (LED) irradiation treatments with conventional anti-cancer drugs on CSC-like oral cancer cells that acquired stemness by ectopic over expression of CD133. To evaluate combined LED irradiation anti-cancer drug effects, we investigated the chemosensitizing effect of 635 nm irradiation on 5-fluorouracil (5FU)-treated KB(CD133+) and KB(Vec) cells, interrogating the underlying molecular mechanisms associated with stemness and apoptosis that are responsible for chemopreventive activity. In addition, combination therapy with LED irradiation and 5-FU treatment was carried out in KB(CD133+) and KB(Vec) cell-inoculated mouse models. LED irradiation of 635 nm inhibited CSC-like properties consistent with a decrease in OCT4 and NANOG protein expression, reducing colony-forming ability. In addition, LED irradiation enhanced 5-FU-induced cytotoxicity and improved 5-FUchemosensitivity in KB(CD133+) via enhancement of apoptosis. These findings were validated in vivo, wherein LED irradiation combined with 5-FU treatment inhibited tumor growth in KB(CD133+)-inoculated mice. Collectively, our results provide novel evidence for 635 nm irradiation-induced 5-FU chemosensitization of CSC in oral cancer. In addition, this research highlights that 635 nm LED irradiation may serve as an adjunct treatment to conventional chemotherapeutic drugs in patients with oral cancer. Article Published Date : Sep 26, 2017

Applications of Light Emitting Diodes in Health Care.

Abstract Title: Applications of Light Emitting Diodes in Health Care. Abstract Source: Ann Biomed Eng. 2017 Sep 25. Epub 2017 Sep 25. PMID: 28948402 Abstract Author(s): Jianfei Dong, Daxi Xiong Article Affiliation: Jianfei Dong Abstract: Light emitting diodes (LEDs) have become the main light sources for general lighting, due to their high lumen efficiency and long life time. Moreover, their high bandwidth and the availability of diverse wavelength contents ranging from ultraviolet to infrared empower them with great controllability in tuning brightness, pulse durations and spectra. These parameters are the essential ingredients of the applications in medical imaging and therapies. Despite the fast advances in both LED technologies and their applications, few reviews have been seen to link the controllable emission properties of LEDs to these applications. The objective of this paper is to bridge this gap by reviewing the main control techniques of LEDs that enable creating enhanced lighting patterns for imaging and generating effective photon doses for photobiomodulation. This paper also provides the basic mechanisms behind the effective LED therapies in treating cutaneous and neurological diseases. The emerging field of optogenetics is also discussed with a focus on the application of LEDs. The multidisciplinary topics reviewed in this paper can help the researchers in LEDs, imaging, light therapy and optogenetics better understand the basic principles in each other's field; and hence to stimulate the application of LEDs in health care. Article Published Date : Sep 24, 2017

LED Phototherapy With Gelatin Sponge Promotes Wound Healing in Mice.

Abstract Title: LED Phototherapy With Gelatin Sponge Promotes Wound Healing in Mice. Abstract Source: Photochem Photobiol. 2017 Aug 1. Epub 2017 Aug 1. PMID: 28763104 Abstract Author(s): Heng Zhang, Shupeng Liu, Xiangfei Yang, Na Chen, Fufei Pang, Zhenyi Chen, Tingyun Wang, Jianshe Zhou, Fuji Ren, Xiaoyin Xu, Taihao Li Article Affiliation: Heng Zhang Abstract: Tiny but highly efficient, a light emitting diode (LED) can power a therapy device, such as a phototherapy device, and, at the same time, decrease the device's size requirements. In this study, a LED phototherapy device was designed to investigate the possible impact on wound healing using a mouse model and a cell line exposed to red and blue light. To enhance wound phototherapy, a gelatin sponge was fabricated. Results showed that the red and blue lights promoted cell growth and wound healing, while the blue light with a gelatin sponge protected the wound from infection in the early stages of wound healing. The LED phototherapy device combined with the gelatin sponge, therefore, has potential significance in clinical application for wound healing. This article is protected by copyright. All rights reserved. Article Published Date : Jul 31, 2017

Curcumin photodynamic effect in the treatment of the induced periodontitis in rats.

Abstract Title: Curcumin photodynamic effect in the treatment of the induced periodontitis in rats. Abstract Source: Lasers Med Sci. 2017 Jul 3. Epub 2017 Jul 3. PMID: 28674790 Abstract Author(s): Letícia Helena Theodoro, Marcio Luiz Ferro-Alves, Mariéllen Longo, Marta Aparecida Alberton Nuernberg, Renata Pironato Ferreira, Adriele Andreati, Edilson Ervolino, Cristiane Duque, Valdir Gouveia Garcia Article Affiliation: Letícia Helena Theodoro Abstract: This study assessed the effect of curcumin as a photosensitizer in antimicrobial photodynamic therapy (aPDT) for the treatment of induced periodontitis in rats. Periodontitis was induced via a ligature around the mandibular first molar on the left side of 96 rats. The ligature was removed 7 days later, and the animals were randomized into four groups: NT, no local treatment; CUR, irrigation with curcumin solution (40 μM); LED, irradiation with a light-emitting diode (LED, InGaN, 465-485 nm, 200 mW/cm(2), 60 s); and aPDT, irrigation with curcumin solution (40 μM) followed by irradiation with LED. Eight animals from each group were euthanized at 7, 15, and 30 days post-treatment. Treatments were assessed using alveolar bone loss (ABL) in the furcation region using histological, histometric, and immunohistochemical analyses. Rats treated with aPDT exhibited less ABL at 7 days compared to the NT group, moderate pattern immunolabeling for osteoprotegerin at 30 days, and a pattern of immunolabeling for RANKL from moderate to low. Treatments resulted in smaller numbers of TRAP-positive cells compared to the NT group. aPDT as monotherapy using curcumin as a photosensitizer and LED as the light source was effective in the treatment of induced periodontitis in rats. Article Published Date : Jul 02, 2017

Pulsed light-emitting diodes for higher phytochemical level in microgreens.

Abstract Title: Pulsed light-emitting diodes for higher phytochemical level in microgreens. Abstract Source: J Agric Food Chem. 2017 Jun 15. Epub 2017 Jun 15. PMID: 28618783 Abstract Author(s): Viktorija Vaštakaitė, Akvilė Viršilė, Aušra Brazaitytė, Giedrė Samuolienė, Julė Jankauskienė, Algirdas Novičkovas, Pavelas Duchovskis Article Affiliation: Viktorija Vaštakaitė Abstract: A novel research of pulsed LED lighting versus continuous lighting was conducted by analyzing phytochemicals levels in microgreens. Red pak choi (Brassica rapa var. chinensis), mustard (Brassica juncea L.) and tatsoi (Brassica rapa var. rosularis) were grown indoors under HPS lamps supplemented with monochromatic (455 nm, 470 nm, 505 nm, 590 nm and 627 nm) LEDs (total PPFD 200±10 µmol m(-2) s(-1); 16 h day(-1)). For pulsed light treatments, the frequencies at 2 Hz, 32 Hz, 256 Hz, and 1024 Hz with a duty cycle of 50% of monochromatic LEDs were applied. The results were compared with those under the continuous light (0 Hz) condition in terms of total phenolic content, anthocyanins and antiradical activity (DPPH). The summarize data suggested that pulsed light affected accumulation of secondary metabolites both positive and negative in microgreens. The significant differences in the response of phytochemicals between pulsed light at several frequencies and continuous light were determined. The most positive effects of 2 Hz, 256 Hz, 1024 Hz for total phenolic compounds in mustard under all wavelengths LEDs were achieved. The LEDs frequencies at 2 Hz and 32 Hz were the most suitable for accumulation of anthocyanins in red pak choi and tatsoi. The highest antiradical activity under the treatments of 32 Hz, 256 Hz and 1024 Hz in mustard, and under the 2 Hz frequency in red pak choi and tatsoi was determined. Article Published Date : Jun 14, 2017

Blue light induced reactive oxygen species from flavin mononucleotide and flavin adenine dinucleotide on lethality of HeLa cells.

Abstract Title: Blue light induced reactive oxygen species from flavin mononucleotide and flavin adenine dinucleotide on lethality of HeLa cells. Abstract Source: J Photochem Photobiol B. 2017 Jun 13 ;173:325-332. Epub 2017 Jun 13. PMID: 28633062 Abstract Author(s): Ming-Yeh Yang, Chih-Jui Chang, Liang-Yü Chen Article Affiliation: Ming-Yeh Yang Abstract: Photodynamic therapy (PDT) is a safe and non-invasive treatment for cancers and microbial infections. Various photosensitizers and light sources have been developed for clinical cancer therapies. Flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD) are the cofactor of enzymes and are used as photosensitizers in this study. Targeting hypoxia and light-triggering reactive oxygen species (ROS) are experimental strategies for poisoning tumor cells in vitro. HeLa cells are committed to apoptosis when treated with FMN or FAD and exposed to visible blue light (the maximum emitted wavelength of blue light is 462nm). Under blue light irradiation at 3.744J/cm(2) (=0.52mW/cm(2) irradiated for 2h), the minimal lethal dose is 3.125μM and the median lethal doses (LD50) for FMN and FAD are 6.5μM and 7.2μM, respectively. Individual exposure to visible blue light irradiation or riboflavin photosensitizers does not produce cytotoxicity and no side effects are observed in this study. The western blotting results also show that an intrinsic apoptosis pathway is activated by the ROS during photolysis of riboflavin analogues. Blue light triggers the cytotoxicity of riboflavins on HeLa cells in vitro. Based on these results, this is a feasible and efficient of PDT with an intrinsic photosensitizer for cancer research. Article Published Date : Jun 12, 2017

Photobiomodulation therapy for the management of radiation-induced dermatitis : A single-institution experience of adjuvant radiotherapy in breast cancer patients after breast conserving surgery.

Abstract Title: Photobiomodulation therapy for the management of radiation-induced dermatitis : A single-institution experience of adjuvant radiotherapy in breast cancer patients after breast conserving surgery. Abstract Source: Strahlenther Onkol. 2017 Jun ;193(6):491-498. Epub 2017 Feb 27. PMID: 28243723 Abstract Author(s): Iosif Strouthos, Georgios Chatzikonstantinou, Nikolaos Tselis, Dimitra Bon, Efstratios Karagiannis, Eleni Zoga, Konstantinos Ferentinos, Julia Maximenko, Vassiliki Nikolettou-Fischer, Nikolaos Zamboglou Article Affiliation: Iosif Strouthos Abstract: BACKGROUND: Radiation therapy (RT) comprises a key component in the treatment of breast cancer. Radiation-induced skin toxicity is the major adverse event experienced by patients; however, radiodermatitis (RD) prevention and management remains trivial. It is proven that photobiomodulation (PBM) therapy using light-emitting diode (LED) increases wound healing and depicts an anti-inflammatory effect. This single-institute study evaluates the beneficial role of PBM-LED in preventing/reducing RD during breast cancer RT. PATIENTS AND METHODS: Of 70 consecutively treated patients, 25 patients were treated with PBM-LED twice a week prior to adjuvant 3D conformal RT after breast-conserving surgery. RD was reported using Common Toxicity Criteria for Adverse Events Version 4.0 and pain intensity using a visual analog scale (VAS). For comparison, a control group (n = 45) received RT without PBM-LED. In addition, a "matched"group (n = 25) was generated from the control group based on propensity for potentially confounding variables. RESULTS: In the PBM group, 22 patients (88%) presented grade 1 and 3 (12%) grade 2 RD. In the control group, 25 patients (55.6%) developed grade 1 reactions, 18 patients (40%) grade 2, and 2 (4.4%) patients grade 3 RD. Concerning pain intensity, 15 patients (60%) of the PBM treatment arm reported no pain, 5 patients (20%) VAS 2, and 5 (20%) VAS 3. In the control group, 13 patients (28.9%) reported no pain, 2 (4.4%) VAS 1, 7 (15.6%) VAS 2, 9 patients (20%) reported VAS 3, 12 (26.7%) patients VAS 4, and 2 (4.4%) patients VAS 5. CONCLUSION: PBM-LED therapy applied prior to RT might be effective in decreasing the incidence and sequelae of radiation-induced skin toxicity in breast cancer patients treated with breast-conserving surgery. Article Published Date : May 31, 2017

Antimicrobial activity of photodynamic therapy in combination with colistin against a pan-drug resistant Acinetobacter baumannii isolated from burn patient.

Abstract Title: Antimicrobial activity of photodynamic therapy in combination with colistin against a pan-drug resistant Acinetobacter baumannii isolated from burn patient. Abstract Source: Photodiagnosis Photodyn Ther. 2017 Jun ;18:1-5. Epub 2017 Jan 12. PMID: 28088439 Abstract Author(s): Ebrahim Boluki, Hossein Kazemian, Hadi Peeridogaheh, Mohammad Yousef Alikhani, Sima Shahabi, Leili Beytollahi, Roghayeh Ghorbanzadeh Article Affiliation: Ebrahim Boluki Abstract: Nosocomially-acquired multi-, extensively-, and pandrug resistant (MDR, XDR, and PDR) strains of microorganisms such as Acinetobacter baumannii remain a serious cause of infection and septic mortality in burn patients. Treatment of patients with nosocomial burn wound infections is often complicated by drug-resistant strains of A. baumannii. Today, many researchers are focusing on the investigation of novel non-antibiotic strategies such as photodynamic therapy (PDT). We report a new PDT strategy that suppresses colistin resistance in PDR A. baumannii by interfering with the expression of a pmrA/pmrB two-component system. In the current study, A. baumannii with a PDR feature isolated from a burn patient was used as a test strain. PDT was carried out using toluidine blue O (TBO) and light-emitting diode (LED) as a photosensitizer and radiation source, respectively. The antimicrobial susceptibility profiles were assessed for cells surviving PDT. The effects of sub-lethal PDT (sPDT) on the expression of the pmrA/pmrB two-component signal transduction system were evaluated by real-time quantitative reverse transcription PCR. Results of drug susceptibly testing (DST) in LED and TBO groups separately showed that the bacteria were resistant to all tested antibiotics, while the DST result of the LED+TBO group showed highly declining bacterial growth when compared with the control group. Reduction in the expression of pmrA and pmrB was observed in the treated strains after sPDT. This represents the first conclusive example of a direct role for the PDT in breaking antibiotic resistance by directly modulating two-component system activity. Article Published Date : May 31, 2017

Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice.

Abstract Title: Low-level light emitting diode therapy promotes long-term functional recovery after experimental stroke in mice. Abstract Source: J Biophotonics. 2017 May 2. Epub 2017 May 2. PMID: 28464523 Abstract Author(s): Hae In Lee, Sae-Won Lee, Nam Gyun Kim, Kyoung-Jun Park, Byung Tae Choi, Yong-Il Shin, Hwa Kyoung Shin Article Affiliation: Hae In Lee Abstract: We aimed to investigate the effects of low-level light emitting diode therapy (LED-T) on the long-term functional outcomes after cerebral ischemia, and the optimal timing of LED-T initiation for achieving suitable functional recovery. Focal cerebral ischemia was induced in mice via photothrombosis. These mice were assigned to a sham-operated (control), ischemic (vehicle), or LED-T group [initiation immediately (acute), 4 days (subacute) or 10 days (delayed) after ischemia, followed by once-daily treatment for 7 days]. Behavioral outcomes were assessed 21 and 28 days post-ischemia, and histopathological analysis was performed 28 days post-ischemia. The acute and subacute LED-T groups showed a significant improvement in motor function up to 28 days post-ischemia, although no brain atrophy recovery was noted. We observed proliferating cells (BrdU(+) ) in the ischemic brain, and significant increases in BrdU(+) /GFAP(+) , BrdU(+) /DCX(+) , BrdU(+) /NeuN(+) , and CD31(+) cells in the subacute LED-T group. However, the BrdU(+) /Iba-1(+) cell count was reduced in the subacute LED-T group. Furthermore, the brain-derived neurotrophic factor (BDNF) was significantly upregulated in the subacute LED-T group. We concluded that LED-T administered during the subacute stage had a positive impact on the long-term functional outcome, probably via neuron and astrocyte proliferation, blood vessel reconstruction, and increased BDNF expression. Picture: The rotarod test for motor coordination showed that acute and subacute LED-T improves long-term functional recovery after cerebral ischemia. Article Published Date : May 01, 2017

Light-Emitting Diode (LED) therapy improves occipital cortex damage by decreasing apoptosis and increasing BDNF-expressing cells in methanol-induced toxicity in rats.

Abstract Title: Light-Emitting Diode (LED) therapy improves occipital cortex damage by decreasing apoptosis and increasing BDNF-expressing cells in methanol-induced toxicity in rats. Abstract Source: Biomed Pharmacother. 2017 May ;89:1320-1330. Epub 2017 Mar 18. PMID: 28320099 Abstract Author(s): Amir Ghanbari, Majid Ghareghani, Kazem Zibara, Hamdallah Delaviz, Elham Ebadi, Mohammad Hossein Jahantab Article Affiliation: Amir Ghanbari Abstract: Methanol-induced retinal toxicity, frequently associated with elevated free radicals and cell edema, is characterized by progressive retinal ganglion cell (RGC) death and vision loss. Previous studies investigated the effect of photomodulation on RGCs, but not the visual cortex. In this study, the effect of 670nm Light-Emitting Diode (LED) therapy on RGCs and visual cortex recovery was investigated in a seven-day methanol-induced retinal toxicity protocol in rats. Methanol administration showed a reduction in the number of RGCs, loss of neurons (neuronal nuclear antigen, NeuN+), activation of glial fibrillary acidic protein (GFAP+) expressing cells, suppression of brain-derived neurotrophic factor (BDNF+) positive cells, increase in apoptosis (caspase 3+) and enhancement of nitric oxide (NO) release in serum and brain. On the other hand, LED therapy significantly reduced RGC death, in comparison to the methanol group. In addition, the number of BDNF positive cells was significantly higher in the visual cortex of LED-treated group, in comparison to methanol-intoxicated and control groups. Moreover, LED therapy caused a significant decrease in cell death (caspase 3+ cells) and a significant reduction in the NO levels, both in serum and brain tissue, in comparison to methanol-intoxicated rats. Overall, LED therapy demonstrated a number of beneficial effects in decreasing oxidative stress and in functional recovery of RGCs and visual cortex. Our data suggest that LED therapy could be a potential condidate as a non-invasive approach for treatment of retinal damage, which needs further clinicl studies. Article Published Date : Apr 30, 2017
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Therapeutic Actions Light-Emitting Diodes (LEDs) Therapy

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Assessing the impact of low level laser therapy (LLLT) on biological systems: a review.

Related Articles Assessing the impact of low level laser therapy (LLLT) on biological systems: a review. Int J Radiat Biol. 2019 Jan 07;:1-24 Authors: Mussttaf RA, Jenkins DFL, Jha AN Abstract PURPOSE: Low level laser therapy (LLLT) in the visible to near infrared spectral band (390-1100 nm) is absorption of laser light at the electronic level, without generation of heat. It may be applied in a wide range of treatments including wound healing, inflammation and pain reduction. Despite its potential beneficial impacts, the use of lasers for therapeutic purposes still remains controversial in mainstream medicine. Whilst taking into account the physical characteristics of different qualities of lasers, this review aims to provide a comprehensive account of the current literature available in the field pertaining to their potential impact at cellular and molecular levels elucidating mechanistic interactions in different mammalian models. The review also aims to focus on the integral approach of the optimal characteristics of LLLT that suit a biological system target to produce the beneficial effect at the cellular and molecular levels. METHODS: Recent research articles were reviewed that explored the interaction of lasers (coherent sources) and LEDs (incoherent sources) at the molecular and cellular levels. RESULTS: It is envisaged that underlying mechanisms of beneficial impact of lasers to patients involves biological processes at the cellular and molecular levels. The biological impact or effects of LLLT at the cellular and molecular level could include cellular viability, proliferation rate, as well as DNA integrity and the repair of damaged DNA. This review summarizes the available information in the literature pertaining to cellular and molecular effects of lasers. CONCLUSIONS: It is suggested that a change in approach is required to understand how to exploit the potential therapeutic modality of lasers whilst minimizing its possible detrimental effects. PMID: 30614743 [PubMed - as supplied by publisher]
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