CYBERMED LIFE - ORGANIC  & NATURAL LIVING

Ultrasound

Ultrasound induces cellular destruction of nasopharyngeal carcinoma cells in the presence of curcumin.

Written by CYBERMED LIFE NEWS
facebook Share on Facebook
Abstract Title:

Ultrasound induces cellular destruction of nasopharyngeal carcinoma cells in the presence of curcumin.

Abstract Source:

Ultrasonics. 2011 Feb;51(2):165-70. Epub 2010 Aug 4. PMID: 20728195

Abstract Author(s):

Xinna Wang, Xinshu Xia, Albert Wingnang Leung, Junyan Xiang, Yuan Jiang, Ping Wang, Jing Xu, Heping Yu, Dingqun Bai, Chuanshan Xu

Article Affiliation:

Department of Photodynamic and Sondynamic Therapy, The Second Affiliated Hospital, The Chongqing Medical University, Chongqing, China.

Abstract:

OBJECTIVES: Curcumin, a natural pigment from the traditional Chinese herb, has shown promise as an efficient enhancer of ultrasound. The present study aims to investigate ultrasound-induced cellular destruction of nasopharyngeal carcinoma cells in the presence of curcumin in vitro.

METHODS: Nasopharyngeal carcinoma cell line CNE2 cells were incubated by 10μm curcumin and then were treated by ultrasound for 8s at the intensity of 0.46W/cm(2). Cytotoxicity was evaluated using MTT assay and light microscopy. Mitochondrial damage was analyzed using a confocal laser scanning microcopy with Rhodamine 123 and ultrastructural changes were observed using a transmission electron microscopy (TEM).

RESULTS: MTT assay showed that cytotoxicity induced by ultrasound treatment alone and curcumin treatment alone was 18.16±2.37% and 24.93±8.30%, respectively. The cytotoxicity induced by the combined treatment of ultrasound and curcumin significantly increased up to 86.67±7.78%. TEM showed that microvillin disappearance, membrane blebbing, chromatin condensation, swollen mitochondria, and mitochondrial myelin-likebody were observed in the cells treated by ultrasound and curcumin together. The significant collapse of mitochondrial membrane potential (MMP) was markedly observed in the CNE2 cells after the combined treatment of curcumin and ultrasound.

CONCLUSIONS: Our findings demonstrated that ultrasound sonication in the presence of curcumin significantly killed the CNE2 cells and induced ultrastructural damage and the dysfunction of mitochondria, suggesting that ultrasound treatment remarkably induced cellular destruction of nasopharyngeal carcinoma cells in the presence of curcumin.


We use cookies on our website. Some of them are essential for the operation of the site, while others help us to improve this site and the user experience (tracking cookies). You can decide for yourself whether you want to allow cookies or not. Please note that if you reject them, you may not be able to use all the functionalities of the site.